Bacteriocinas

Curr Microbiol (2011) 62:1017–1022
DOI 10.1007/s00284-010-9814-z

Antimicrobial Activity of Bacillus amyloliquefaciens LBM 5006 is Enhanced in the Presence of Escherichia coli
Lisianne Benitez • AnaPaula Correa
Daniel Daroit • Adriano Brandelli

•

Received: 22 September 2010 / Accepted: 2 November 2010 / Published online: 21 November 2010
Ó Springer Science+Business Media, LLC 2010

Abstract Increased antimicrobial activity was observed when Bacillus amyloliquefaciens LBM 5006 strain was cultivated in the presence of thermally inactivated cells of
Escherichia coli, but not with Staphylococcus aureus,
Listeria monocytogenes, or Bacillus cereus. E. coli also enhanced the antimicrobial activity when it was added to the medium in the form of living cells or as cell debris after cellular fractionation. No inducing activity was observed with addition of cell-free supernatant of E. coli cultures, suggesting that inducing factor is associated to the cells.
Polyacrylamide gel electrophoresis revealed that additional peptide bands are secreted when B. amyloliquefaciens was cultivated in the presence of cell debris of E. coli. These results suggest that the presence of intact or inactivated
E. coli enhanced the synthesis of antimicrobial peptides by
B. amyloliquefaciens LBM 5006.

L. Benitez
´
Bioquımica e Microbiologia, Universidade Federal do Rio
Grande do Sul, Porto Alegre, Brazil
A. Correa
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
D. Daroit
Universidade Federal do Rio Grande do Sul,
Porto Alegre, Brazil
A. Brandelli (&)
ˆ
Departamento de Ciencia de Alimentos, Universidade Federal do
Rio Grande do Sul, Porto Alegre, Brazil e-mail: [e-mail]

Introduction
Bacteria of the genus Bacillus can produce a large number of antimicrobial peptides with different chemical structures, such as bacteriocins, bacteriocin-like substances
(BLS), and lipopeptides [13, 23]. Although most of