16S rrna

Phylogenetic Diversity of Bacteria Associated with the Marine Sponge Rhopaloeides odorabile
Nicole S. Webster, Kate J. Wilson, Linda L. Blackall and Russell T. Hill Appl. Environ. Microbiol. 2001, 67(1):434. DOI: 10.1128/AEM.67.1.434-444.2001.

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APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Jan. 2001, p. 434–444 0099-2240/01/$04.00 0 DOI: 10.1128/AEM.67.1.434–444.2001 Copyright © 2001, American Society for Microbiology. All Rights Reserved.

Vol. 67, No. 1

Phylogenetic Diversity of Bacteria Associated with the Marine Sponge Rhopaloeides odorabile†
NICOLE S. WEBSTER,1 KATE J. WILSON,1 LINDA L. BLACKALL,2
AND

RUSSELL T. HILL1,3*

Australian Institute of Marine Science, Townsville, Queensland, Australia 48101; Department of Microbiology and Parasitology, University of Queensland, St. Lucia, Queensland, Australia 40672; and Centre of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 212023
Received 20 June 2000/Accepted 19 September 2000

Molecular techniques were employed to document the microbial diversity associated with the marine sponge Rhopaloeides odorabile. The phylogenetic affiliation of sponge-associated bacteria was assessed by 16S rRNA sequencing of cloned DNA fragments. Fluorescence in situ hybridization (FISH) was used to confirm the presence of the predominant groups indicated by 16S rDNA analysis. The community