Como pipetar

Gilson guide to pipetting

Gilson guide to pipetting

Order Online at: www.pipetman.com Or Contact Gilson at:
1-800-GILSON1

LT303409-01 Copyright © 2005 Gilson, Inc. Allrights reserved.Printed in the USA.

$20 US

Second Edition

Gilson, Inc. World Headquarters
P.O. Box 628098
Middleton, WI 53562-8098 USA
Telephone: [phone] or 800-445-7661
Fax: [phone]

Second Edition

Louis Pasteur the father of modern biology
1822-1895

Over a century ago, Louis Pasteur gave glass pipettes a new form to prevent liquid reflux. The Pasteur pipette is still in use today.
The next significant improvement on pipettes came in the late 50’s with the introduction of a hand-held, piston-operated pipette as a safe alternative to potentially dangerous mouth pipetting. The first hand-held pipettes had a pre-established volume setting (fixed volume pipettes). Further improvement came with the introduction of a more flexible, stepper volume setting
(variable volume pipettes).

Pipetting since Louis Pasteur
In 1972, Dr. Warren Gilson introduced a high precision pipette which could be adjusted for any volume within its range… even fractional (continuously adjustable). Its originality… to avoid error, the selected volume was clearly displayed on the Gilson pipette (direct digital readout).
Twenty-five years later, Gilson precision pipettes are still the world standard for accuracy, precision and reliability.

Photo courtesy of the Institut Pasteur.

Pipette guiderev11012006.indd 1

18/07/2007 16:45:08

Chapter 1

Selecting the best pipette for your application
Consider the physical properties of your sample - - - - - - - - - - - - - - - - - - - - - - - - - - n n Viscous samples

n

Radioactive or corrosive samples

n

17

Aqueous samples

DNA, RNA, biological samples

The working principle of air-displacement and of positive-displacement pipettes - -

18

L earning more… A ll air-displacement