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Asian Pacific Journal of Tropical Biomedicine (2012)1-6

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Document heading

Statistical optimization of glutaminase production from Lactobacillus casei using response surface methodology
Sajitha Nagamony*, Lakshminarayanan Ramasamy1, Vasanthabharathi venkataraman2, S. Jayalakshmi Singaram3
CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608 502, Tamilnadu, India.

ARTICLE INFO

ABSTRACT

Article history:
Received 15 April 2011
Received in revised form 27 April
Accepted 28 June 2011
Available online 28 June 2011

Objective: To isolate and optimize the glutaminase producing bacteria using Response Surface
Methodology. Methods: In the present study, the water, sediment samples and mangrove detritus were collected from the vellar estuary (Tamil Nadu, India) and the potential strains of glutamines production were screened in minimal medium plates. The optimization of glutaminase enzyme was done by response surface methodology. Result: Totally 25 strains were collected. Among them
Lactobacillus casei in was found to be the potential strain. Hence this potential strain was used for further optimization using Response Surface Methodology, partial purification using ammonium sulphate precipitation, characterization, stability, antimicrobial and antioxidant application. The stability of glutaminase from Lactobacillus casei was studied and better stability was observed at pH 7 to 8. The thermal stability was observed at 30°C to 40°C. The SDS PAGE of glutaminase enzyme shows two different molecular weights. The molecular weight of partially purified glutaminase enzyme was 23kDa and 34kDa. The glutaminase enzyme shows moderate antibacterial activity. The glutaminase enzyme showed antioxidant activity. Conclusion: Thus the present study revealed that this