Puc 19 e 18
The map shows enzymes that cut pUC18/19 DNA once. The coordinates refer to the position of first nucleotide in each recognition sequence.
The exact position of genetic elements is shown on the map (termination codons included). The bla gene nucleotides 2486-2418 (complementary strand) code for a signal peptide. The LacZ polypeptide corresponding to wt beta-galactosidase and essential for blue/white screening ends at nt position 236 (compl. strand); another 30 codons in the same reading frame are derived from pBR322. The indicated rep region is sufficient to promote