Imunofluorescencia

Thermo Scientific Pierce Antibody Immunostaining Guide

Immunostaining

Design, visualize and detect
Primary Antibodies • Secondary Antibodies • Staining Dyes • Kits

Table of Contents Introduction The Cell Mammalian Cell Type Choices Immunohistochemistry Immunofluorescence Secondary Antibodies Primary Antibodies by Cellular Structures by Research Areas by Cell Signaling by Biological Processes 28-31 32-43 44-59 60-76 Page 1-3 4-5 6-8 9-12 13-22 23-27

Thermo Scientific Pierce Antibody Immunostaining Guide

Left: Detection of mouse anti-α-tubulin in an A549 cell in Telophase with Thermo Scientific DyLight Dye 550-GAM. Chromosomes (orange) at the poles become diffuse, while nuclei (blue) divide into two future cells.

Introduction
1

Immunofluorescence (IF) and immunohistochemistry (IHC) are two methods commonly used to detect proteins in a cellular context. Immunofluorescent detection of proteins can be performed on both fixed cells in culture and on paraffin or frozen tissue sections. The advantages of using IF to detect cellular proteins includes the ability to visualize the subcellular location of protein(s) of interest, assess both protein expression and post-translational modifications, and design multiplex experiments. When IF detection is extended to tissues sections (IHC), a higher level of resolution is achieved because researchers are analyzing target protein(s) in a near physiological state, making it ideal for assessing normal and disease tissues.

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Introduction

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We have over 30,000 antibodies in 42 research areas. Thermo Scientific Pierce Antibodies are developed for a wide variety of application needs. Our website enables you to easily search by protein target and then filter by the specific assays that interest you. All of our antibodies are validated in the stated