Esbl

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Detection of Extended-Spectrum β -Lactamase and Klebsiella pneumoniae Carbapenemase Genes Directly from Blood Cultures by Use of a Nucleic Acid Microarray
Joel T. Fishbain, Oleg Sinyavskiy, Kathleen Riederer, Andrea M. Hujer and Robert A. Bonomo J. Clin. Microbiol. 2012, 50(9):2901. DOI: 10.1128/JCM.01023-12. Published Ahead of Print 20 June 2012.

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Detection of Extended-Spectrum -Lactamase and Klebsiella pneumoniae Carbapenemase Genes Directly from Blood Cultures by Use of a Nucleic Acid Microarray
Downloaded from http://jcm.asm.org/ on January 14, 2013 by UNIVERSIDADE DE SÃO PAULO
Joel T. Fishbain,a Oleg Sinyavskiy,a Kathleen Riederer,a Andrea M. Hujer,b,c and Robert A. Bonomob,c,d,e
Departments of Medicine and Graduate Medical Education, St. John Hospital and Medical Center, Detroit, Michigan, USAa; Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USAb; Research Service, Louis Stokes Cleveland Veterans Affairs Medical Center, Cleveland, Ohio, USAc; and Departments of Molecular Biology and Microbiologyd and Pharmacology,e Case Western Reserve University School of Medicine, Cleveland, Ohio USA

The growing crisis of multidrug-resistant (MDR) Gram-negative bacteria requires that current technologies permit the rapid detection of extended-spectrum -lactamase (blaESBL) and Klebsiella pneumoniae carbapenemase (blaKPC) genes. In the present study, we assessed the performance characteristics of a commercially available nucleic acid microarray system for the detection of blaESBL and blaKPC genes directly from positive blood cultures. Using blood cultures (BCs) that contained Gram-negative bacilli identified by Gram staining, we isolated bacterial DNA using spin columns (BC-C) and rapid water lysis (BC-W). Twenty

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