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Pesq. Vet. Bras. 18(2):83-90, abr./jun. 1998

Caracterização preliminar de amostras do vírus da Diarréia Viral Bovina (BVDV) isoladas no Brasil1
Sônia A. Botton2, Laura Helena V.G. Gil2, Adriana M. da Silva2, Eduardo F. Flores3,4, Rudi Weiblen3, E. Maristela Pituco5, Paulo M. Roehe6, Valéria Moojen7 e Ana Cristina Wendelstein7
ABSTRACT.- Botton S.A., Gil L.H.V.G., Silva A.M., Flores E.F.,Weiblen R., Pituco E.M., Roehe P.M., Moojen V. & Wendelstein, A.C. 1998. [Preliminary characterization of brazilian isolates of bovine viral diarrhea virus (BVDV).] Caracterização preliminar de amostras do vírus da Diarréia Viral Bovina (BVDV) isoladas no Brasil. Pesquisa Veterinária Brasileira, 18(2)83-90. Depto Medicina Veterinária Preventiva, Microbiologia e Parasitologia, Universidade Federal deSanta Maria, Santa Maria, RS 97015-900, Brazil. This article reports the preliminary characterization of 19 Brazilian bovine viral diarrhea virus (BVDV) isolates, regarding the biological, antigenic and molecular properties. Eleven viruses were isolated from bovine fetuses, six were obtained from blood of animals from herds with reproductive problems, and two were isolated from clinical cases ofgastroenteric disease. The clinical cases affected young animals and were characterized by diarrhea, oronasal and digestive erosions and ulceration, and occasional digestive bleeding and vulvar petechial hemorrhage. Sixteen isolates (84.2%), including those obtained from fetuses and clinical cases, were of the non-cytopathic (ncp) biotype. Replication of three isolates (15.8%) in tissue culture wascharacterized by appearance of cellular vacuolation and progressive destruction of the monolayers. Analysis of these isolates after cloning revealed a mixed population of cytopathic (cp) and non-cytopathic viruses. Analysis of viral polypeptides by SDS-PAGE followed by “Western immunoblot” revealed the production of the nonstructural protein NS3/p80 in cells infected with the cp viruses. Incontrast, generation of NS3/p80 was not observed in cells infected with the ncp isolates, which only expressed the precursor polypeptide NS23/p125. Analysis of reactivity with a panel of 15 monoclonal antibodies (MAbs) revealed a marked antigenic variability among the isolates, mainly in the envelope glycoprotein E2/ gp53. Although one MAb to this glycoprotein recognized 18 isolates (94.7%), the othernine E2/ gp53 MAbs recognized zero to 57.9% of the isolates. The marked antigenic diversity observed among the brazilian BVDV isolates may have important implications on diagnosis and immunization strategies.
INDEX TERMS: Bovine viral diarrhea virus, BVDV, biotypes, antigenic diversity.
1Aceito

para publicação em 18 de março de 1998.

Parte da tese de Mestrado dos dois primeiros autores.Programa de PósGraduação em Medicina Veterinária, Universidade Federal de Santa Maria (UFSM).
2Mestrando

em Medicina Veterinária, UFSM.

de Medicina Veterinária Preventiva (DMVP), Microbiologia e Parasitologia (DMP), UFSM.
4Autor para correspondência:DMVP/UFSM, Santa Maria, RS 97015-900. Fax:(055)220-8257. E-mail: Flores@ccr.ufsm.br. Bolsista do CNPq (352386/ 96-7). 5Instituto 6Centro3Departamento

Biológico de São Paulo, São Paulo, SP

de Pesquisas Veterinárias Desidério Finamor (CPVDF), Eldorado do Sul, RS, e Departamento de Microbiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS.
7Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS.

RESUMO.- O presente artigo relata a caracterização inicial de 19 amostras dovírus da Diarréia Viral Bovina (BVDV) isoladas no Brasil, com relação a aspectos biológicos, antigênicos e moleculares. Onze amostras foram isoladas de fetos bovinos, seis foram obtidas do sangue de animais clinicamente saudáveis de rebanhos com problemas reprodutivos e duas amostras foram isoladas de casos clínicos de enfermidade gastrentérica. Os casos de doença entérica afetaram animais jovens...
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