Conditional Dicer Gene
Depletion in the
MARTINS, P. A. C.; BOURAJJAJ, M.; GLADKA, M.;
KORTLAND, M.; OORT,R. J. V.; PINTO, Y. M.;MOLKENTIN, J. D.; WINDT, L. J.
Circulation, v. 118, n. 15, oct. 2008
American Heart Association
Aluna: Dayana P. P. Siqueira
(18 to 24 nucleotides long)
AMBROS, 2003; FARH, et al., 2005; PASQUINELLI, HUNTER, BRACHT, 2005; CULLEN, 2004.
BARTEL, 2004; BENTWICH et al., 2005; BEREZIKOV et al., 2005.
protein expression by
May regulateup 1/3 of the
Fundamental role in
Trigger loss of Dicer in the postnatal myocardiumthrough the use of a tamoxifen-inducible Cre
recombinase expressed in murine heart cells.
Mice were treated with:
• vehicle or tamoxifen (20mg/Kg per day)
•intraperitoneal injections for consecutive 5 days
Proteins were extracted from mouse cardiac tissue
Dicer (rabbit polyclonal)
Irx5 (mouse monoclonal)
Kcnd2 (rabbit polyclonal)
Histological Analysis and Immunofluorescence Microscopy
Hearts were arrested in diastole
Perfusion-fixed with 4%paraformaldehyde
Embedded in parafin
Sectioned at 5µm
routine histological analysis
detection of fibrillar collagen
visualize and quantify the cross-sectional area ofcardiomyocytes
visualize and quantify the number of capillaries per
Were performed on mice anesthetized with isoflurane
1 week and 4 weeks...
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