Como pipetar

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Gilson guide to pipetting

Gilson
guide
to pipetting

Order Online at:
www.pipetman.com

Or Contact Gilson at:
1-800-GILSON1

LT303409-01 Copyright © 2005 Gilson, Inc. Allrights reserved.Printed in the USA.

$20 US

Second Edition

Gilson, Inc. World Headquarters
P.O. Box 628098
Middleton, WI 53562-8098 USA
Telephone: 608-836-1551 or 800-445-7661
Fax: 608-831-4451

SecondEdition

Louis Pasteur
the father of modern biology
1822-1895

Over a century ago, Louis Pasteur gave
glass pipettes a new form to prevent
liquid reflux. The Pasteur pipette is still in
use today.
The next significant improvement
on pipettes came in the late 50’s
with the introduction of a hand-held,
piston-operated pipette as a safe
alternative to potentially dangerous
mouthpipetting. The first hand-held
pipettes had a pre-established volume
setting (fixed volume pipettes). Further
improvement came with the introduction
of a more flexible, stepper volume setting
(variable volume pipettes).

Pipetting since Louis Pasteur
In 1972, Dr. Warren Gilson introduced
a high precision pipette which could
be adjusted for any volume within its
range… even fractional(continuously
adjustable). Its originality… to avoid
error, the selected volume was clearly
displayed on the Gilson pipette (direct
digital readout).
Twenty-five years later, Gilson precision
pipettes are still the world standard for
accuracy, precision and reliability.

Photo courtesy of the Institut Pasteur.

Pipette guiderev11012006.indd 1

18/07/2007 16:45:08

Chapter 1

Selectingthe best pipette for your application
Consider the physical properties of your sample - - - - - - - - - - - - - - - - - - - - - - - - - - n
n

Viscous samples

n

Radioactive or corrosive samples

n

17

Aqueous samples

DNA, RNA, biological samples

The working principle of air-displacement and of positive-displacement pipettes - -

18

L earning more… A ll air-displacementpipettes are not the same
L earning more… Mistake-free pipetting
Consider the volume of liquid you want to transfer - - - - - - - - - - - - - - - - - - - - - - - - n

Volume range of Pipetman and Pipetman Ultra

n

Volume range of Microman

n

14

Volume range of Distriman

L earning more… How do I read the volume?

Chapter 2

Selecting the right tip for your pipette
Evaluatingtips - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 17
L earning more… More than meets the eye
Choosing the best tip for your application - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 18
n

Racked tips

n

Sterilized tips

n

Filter tips

L earning more… How to prevent aerosol contamination?

Chapter 3Correct operation

Organize your work station - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 21
Fit a disposable pipette tip - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 22
L earning more… How to mount tips on a Pipetman Ultra multichannel pipette?
Adjust the volume setting - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - 24
Follow the instructions for forward or reverse mode pipetting- - - - - - - - - - - - - - - - - 25
L earning more… Pre-rinsing
Eject the used tip and store the pipette in an upright position - - - - - - - - - - - - - - - - - 32
L earning more… Why do I need a pipette holder?

Pipette guiderev11012006.indd 2

18/07/2007 16:45:08 Chapter 4

Preventing contamination
Types of contamination and how to prevent them - - - - - - - - - - - - - - - - - - - - - - - - - - 35
n

Personal exposure

n

Pipette-to-sample

n

Sample-to-pipette

n

Sample-to-sample

Decontaminating your Pipetman - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 36

Chapter 5

Caring for your pipette
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