Atividade microbiana

IN VITRO ANTIMICROBIAL ACTIVITY OF FOUR FICUS CARICA LATEX FRACTIONS AGAINST RESISTANT HUMAN PATHOGENS (ANTIMICROBIAL ACTIVITY OF FICUS CARICA LATEX)
HOUDA LAZREG AREF1*, KARIMA BEL HADJ SALAH2, JEAN PIERRE CHAUMONT3, ABDELWAHEB FEKIH4, MAHJOUB AOUNI2 AND KHALED SAID1
Laboratoire de Génétique : Biodiversité et valorisation des bio ressources, Institut Supérieur de Biotechnologie, 5000 Monastir, Tunisia 2 Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Faculté de Pharmacie 5000 Monastir, Tunisia 3 Laboratoire de Botanique et cryptogamie, Faculté de Pharmacie Besançon 25000 cedex France 4 Laboratoire de Chimie, 03/UR/1202, Faculté de Médecine Dentaire, 5000 Monastir, Tunisie ABSTRACT Methanolic, hexanoïc, chloroformic and ethyl acetate extracts of Ficus carica latex were investigated for their in vitro antimicrobial proprieties against five bacteria species and seven strains of fungi. The green fruit latex was collected from Chott Mariam Souse, Middle East coast of Tunisia. The antimicrobial activity of the extracts was evaluated and based respectively on the inhibition zone using the disc – diffusion assay, minimal inhibition concentration (MIC) for bacterial testing and the method by calculating inhibition percentage (I%) for fungiinhibiting activities. The methanolic extract had no effect against bacteria except for Proteus mirabilis while the ethyl acetate extract had inhibition effect on the multiplication of five bacteria species (Enterococcus fecalis, Citobacter freundei, Pseudomonas aeruginosa, Echerchia coli and Proteus mirabilis). For the opportunist pathogenic yeasts, ethyl acetate and chlorophormic fractions showed a very strong inhibition (100%); methanolic fraction had a total inhibition against Candida albicans (100%) at a concentration of 500µg/ml and a negative effect against Cryptococcus neoformans. Microsporum canis was strongly inhibited with methanolic extract (75%) and totally with ethyl acetate extract at a